Review



rnascope 2 5 hd detection reagent red kit  (Advanced Cell Diagnostics Inc)


Bioz Verified Symbol Advanced Cell Diagnostics Inc is a verified supplier
Bioz Manufacturer Symbol Advanced Cell Diagnostics Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Advanced Cell Diagnostics Inc rnascope 2 5 hd detection reagent red kit
    Rnascope 2 5 Hd Detection Reagent Red Kit, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 99/100, based on 618 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope%C2%AE+2%2E5+hd+detection+reagent-red+kit/RNAscope+2%2E5+HD+Detection+Reagents-RED/pm42745355-109-6-12
    Average 99 stars, based on 618 article reviews
    rnascope 2 5 hd detection reagent red kit - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    RNA Expression:

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice
    Article Snippet: The RNAscope ® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice.
    Article Snippet: RNAscope in situ hybridization The RNAscope® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: CCDC89 is required for optimal sperm motility and male fertility in mammals.
    Article Snippet: Slides were visualized with ImmPACT AEC peroxidase substrate (Vector Laboratories) before counterstaining with Mayer’s haematoxylin, then mounted with Aquatex mounting medium (Merck KGaA, Germany).

    In Situ Hybridization:

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice
    Article Snippet: The RNAscope ® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice.
    Article Snippet: RNAscope in situ hybridization The RNAscope® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: CCDC89 is required for optimal sperm motility and male fertility in mammals.
    Article Snippet: Slides were visualized with ImmPACT AEC peroxidase substrate (Vector Laboratories) before counterstaining with Mayer’s haematoxylin, then mounted with Aquatex mounting medium (Merck KGaA, Germany).

    RNAscope:

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice
    Article Snippet: The RNAscope ® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice.
    Article Snippet: RNAscope in situ hybridization The RNAscope® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: CCDC89 is required for optimal sperm motility and male fertility in mammals.
    Article Snippet: Slides were visualized with ImmPACT AEC peroxidase substrate (Vector Laboratories) before counterstaining with Mayer’s haematoxylin, then mounted with Aquatex mounting medium (Merck KGaA, Germany).

    Expressing:

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice
    Article Snippet: The RNAscope ® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice.
    Article Snippet: RNAscope in situ hybridization The RNAscope® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: CCDC89 is required for optimal sperm motility and male fertility in mammals.
    Article Snippet: Slides were visualized with ImmPACT AEC peroxidase substrate (Vector Laboratories) before counterstaining with Mayer’s haematoxylin, then mounted with Aquatex mounting medium (Merck KGaA, Germany).

    Gene Expression:

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice
    Article Snippet: The RNAscope ® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice.
    Article Snippet: RNAscope in situ hybridization The RNAscope® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: CCDC89 is required for optimal sperm motility and male fertility in mammals.
    Article Snippet: Slides were visualized with ImmPACT AEC peroxidase substrate (Vector Laboratories) before counterstaining with Mayer’s haematoxylin, then mounted with Aquatex mounting medium (Merck KGaA, Germany).

    Marker:

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice
    Article Snippet: The RNAscope ® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice.
    Article Snippet: RNAscope in situ hybridization The RNAscope® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: CCDC89 is required for optimal sperm motility and male fertility in mammals.
    Article Snippet: Slides were visualized with ImmPACT AEC peroxidase substrate (Vector Laboratories) before counterstaining with Mayer’s haematoxylin, then mounted with Aquatex mounting medium (Merck KGaA, Germany).

    Staining:

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice
    Article Snippet: The RNAscope ® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice.
    Article Snippet: RNAscope in situ hybridization The RNAscope® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: CCDC89 is required for optimal sperm motility and male fertility in mammals.
    Article Snippet: Slides were visualized with ImmPACT AEC peroxidase substrate (Vector Laboratories) before counterstaining with Mayer’s haematoxylin, then mounted with Aquatex mounting medium (Merck KGaA, Germany).

    Software:

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice
    Article Snippet: The RNAscope ® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice.
    Article Snippet: RNAscope in situ hybridization The RNAscope® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: CCDC89 is required for optimal sperm motility and male fertility in mammals.
    Article Snippet: Slides were visualized with ImmPACT AEC peroxidase substrate (Vector Laboratories) before counterstaining with Mayer’s haematoxylin, then mounted with Aquatex mounting medium (Merck KGaA, Germany).

    Infection:

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice
    Article Snippet: The RNAscope ® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice.
    Article Snippet: RNAscope in situ hybridization The RNAscope® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: CCDC89 is required for optimal sperm motility and male fertility in mammals.
    Article Snippet: Slides were visualized with ImmPACT AEC peroxidase substrate (Vector Laboratories) before counterstaining with Mayer’s haematoxylin, then mounted with Aquatex mounting medium (Merck KGaA, Germany).

    Produced:

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice
    Article Snippet: The RNAscope ® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: Excitatory neuron-prone prion propagation and excitatory neuronal loss in prion-infected mice.
    Article Snippet: RNAscope in situ hybridization The RNAscope® 2.5 HD Detection Reagent-RED kit (#322360, ACDbio, Newark, CA, USA) was used for RNAscope in situ hybridization following the manufacturer’s instructions.

    Article Title: CCDC89 is required for optimal sperm motility and male fertility in mammals.
    Article Snippet: Slides were visualized with ImmPACT AEC peroxidase substrate (Vector Laboratories) before counterstaining with Mayer’s haematoxylin, then mounted with Aquatex mounting medium (Merck KGaA, Germany).



    Similar Products

    99
    Advanced Cell Diagnostics Inc rnascope 2 5 hd detection reagent red kit
    Rnascope 2 5 Hd Detection Reagent Red Kit, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope%C2%AE+2%2E5+hd+detection+reagent-red+kit/RNAscope+2%2E5+HD+Detection+Reagents-RED/pm42745355-109-6-12
    Average 99 stars, based on 1 article reviews
    rnascope 2 5 hd detection reagent red kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Advanced Cell Diagnostics Inc rnascope 2 5 hd assay red kit
    Rnascope 2 5 Hd Assay Red Kit, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope%C2%AE+2%2E5+hd+detection+reagent-red+kit/RNAscope+2%2E5+HD+Detection+Reagents-RED/pm42727582-379-12-17
    Average 99 stars, based on 1 article reviews
    rnascope 2 5 hd assay red kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Advanced Cell Diagnostics Inc rnascope red detection kit
    Rnascope Red Detection Kit, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope%C2%AE+2%2E5+hd+detection+reagent-red+kit/RNAscope+2%2E5+HD+Detection+Reagents-RED/pm42635887-56-16-25
    Average 99 stars, based on 1 article reviews
    rnascope red detection kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Advanced Cell Diagnostics Inc rnascope kit
    (A) Depletion of Fto in the NAcSh using microinjected AAV-Cre/GFP. <t>RNAscope</t> with Fto probe and Fto/GFP imaging was performed on brain sections from Fto fl/fl mice microinjected with AAV-Cre/GFP or AAV-GFP in the NAcSh after morphine CPP study, as described in the Methods. AAV-Cre: AAV5-CMV-Cre/GFP; AAV-GFP: AAV5-CMV-GFP; core: NAc core; shell: NAc shell (NAcSh). (B) Schematic of CPP. (C) Reduced morphine CPP by disrupting Fto in the NAcSh via AAV-Cre in Fto fl/fl mice. Fto fl/fl mice were bilaterally microinjected in the NAcSh with AAV-Cre/GFP or AAV-GFP. Morphine CPP was performed as shown in five weeks post-injection. **: p < 0.01, two-tailed Student t -test. (D) No significant changes in morphine CPP by disrupting both Fto and Pcif1 in the NAcSh in Fto fl/fl ; Pcif1 fl/fl mice. Fto fl/fl ; Pcif1 fl/fl mice were microinjected in the NAcSh with AAV-Cre/GFP or AAV-GFP. Morphine CPP was performed as shown in five weeks post-injection. n.s.: No statistical significance, two-tailed Student t -test. (E) No significant changes in morphine CPP by disrupting Pcif1 in the NAcSh in Pcif1 fl/fl mice. Pcif1 fl/fl mice were microinjected in the NAcSh with AAV-Cre/GFP or AAV-GFP. Morphine CPP was performed as shown in five weeks post-injection. p = 0.1024, no statistical significance, two-tailed Student t -test.
    Rnascope Kit, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope%C2%AE+2%2E5+hd+detection+reagent-red+kit/RNAscope+2%2E5+HD+Detection+Reagents-RED/bio_rxiv__64898__2026__08__06__743062-236-6-8
    Average 99 stars, based on 1 article reviews
    rnascope kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Advanced Cell Diagnostics Inc rnascope 2 5 hd assay red detection kit
    (A) Depletion of Fto in the NAcSh using microinjected AAV-Cre/GFP. <t>RNAscope</t> with Fto probe and Fto/GFP imaging was performed on brain sections from Fto fl/fl mice microinjected with AAV-Cre/GFP or AAV-GFP in the NAcSh after morphine CPP study, as described in the Methods. AAV-Cre: AAV5-CMV-Cre/GFP; AAV-GFP: AAV5-CMV-GFP; core: NAc core; shell: NAc shell (NAcSh). (B) Schematic of CPP. (C) Reduced morphine CPP by disrupting Fto in the NAcSh via AAV-Cre in Fto fl/fl mice. Fto fl/fl mice were bilaterally microinjected in the NAcSh with AAV-Cre/GFP or AAV-GFP. Morphine CPP was performed as shown in five weeks post-injection. **: p < 0.01, two-tailed Student t -test. (D) No significant changes in morphine CPP by disrupting both Fto and Pcif1 in the NAcSh in Fto fl/fl ; Pcif1 fl/fl mice. Fto fl/fl ; Pcif1 fl/fl mice were microinjected in the NAcSh with AAV-Cre/GFP or AAV-GFP. Morphine CPP was performed as shown in five weeks post-injection. n.s.: No statistical significance, two-tailed Student t -test. (E) No significant changes in morphine CPP by disrupting Pcif1 in the NAcSh in Pcif1 fl/fl mice. Pcif1 fl/fl mice were microinjected in the NAcSh with AAV-Cre/GFP or AAV-GFP. Morphine CPP was performed as shown in five weeks post-injection. p = 0.1024, no statistical significance, two-tailed Student t -test.
    Rnascope 2 5 Hd Assay Red Detection Kit, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope%C2%AE+2%2E5+hd+detection+reagent-red+kit/RNAscope+2%2E5+HD+Detection+Reagents-RED/pmc13379590-451-6-12
    Average 99 stars, based on 1 article reviews
    rnascope 2 5 hd assay red detection kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Advanced Cell Diagnostics Inc rnascope 2 5 hd detection kit
    (A) Depletion of Fto in the NAcSh using microinjected AAV-Cre/GFP. <t>RNAscope</t> with Fto probe and Fto/GFP imaging was performed on brain sections from Fto fl/fl mice microinjected with AAV-Cre/GFP or AAV-GFP in the NAcSh after morphine CPP study, as described in the Methods. AAV-Cre: AAV5-CMV-Cre/GFP; AAV-GFP: AAV5-CMV-GFP; core: NAc core; shell: NAc shell (NAcSh). (B) Schematic of CPP. (C) Reduced morphine CPP by disrupting Fto in the NAcSh via AAV-Cre in Fto fl/fl mice. Fto fl/fl mice were bilaterally microinjected in the NAcSh with AAV-Cre/GFP or AAV-GFP. Morphine CPP was performed as shown in five weeks post-injection. **: p < 0.01, two-tailed Student t -test. (D) No significant changes in morphine CPP by disrupting both Fto and Pcif1 in the NAcSh in Fto fl/fl ; Pcif1 fl/fl mice. Fto fl/fl ; Pcif1 fl/fl mice were microinjected in the NAcSh with AAV-Cre/GFP or AAV-GFP. Morphine CPP was performed as shown in five weeks post-injection. n.s.: No statistical significance, two-tailed Student t -test. (E) No significant changes in morphine CPP by disrupting Pcif1 in the NAcSh in Pcif1 fl/fl mice. Pcif1 fl/fl mice were microinjected in the NAcSh with AAV-Cre/GFP or AAV-GFP. Morphine CPP was performed as shown in five weeks post-injection. p = 0.1024, no statistical significance, two-tailed Student t -test.
    Rnascope 2 5 Hd Detection Kit, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope%C2%AE+2%2E5+hd+detection+reagent-red+kit/RNAscope+2%2E5+HD+Detection+Reagents-RED/pm41875882-419-15-12
    Average 99 stars, based on 1 article reviews
    rnascope 2 5 hd detection kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Advanced Cell Diagnostics Inc rnascope 2 5 hd reagent kit red
    (A) Depletion of Fto in the NAcSh using microinjected AAV-Cre/GFP. <t>RNAscope</t> with Fto probe and Fto/GFP imaging was performed on brain sections from Fto fl/fl mice microinjected with AAV-Cre/GFP or AAV-GFP in the NAcSh after morphine CPP study, as described in the Methods. AAV-Cre: AAV5-CMV-Cre/GFP; AAV-GFP: AAV5-CMV-GFP; core: NAc core; shell: NAc shell (NAcSh). (B) Schematic of CPP. (C) Reduced morphine CPP by disrupting Fto in the NAcSh via AAV-Cre in Fto fl/fl mice. Fto fl/fl mice were bilaterally microinjected in the NAcSh with AAV-Cre/GFP or AAV-GFP. Morphine CPP was performed as shown in five weeks post-injection. **: p < 0.01, two-tailed Student t -test. (D) No significant changes in morphine CPP by disrupting both Fto and Pcif1 in the NAcSh in Fto fl/fl ; Pcif1 fl/fl mice. Fto fl/fl ; Pcif1 fl/fl mice were microinjected in the NAcSh with AAV-Cre/GFP or AAV-GFP. Morphine CPP was performed as shown in five weeks post-injection. n.s.: No statistical significance, two-tailed Student t -test. (E) No significant changes in morphine CPP by disrupting Pcif1 in the NAcSh in Pcif1 fl/fl mice. Pcif1 fl/fl mice were microinjected in the NAcSh with AAV-Cre/GFP or AAV-GFP. Morphine CPP was performed as shown in five weeks post-injection. p = 0.1024, no statistical significance, two-tailed Student t -test.
    Rnascope 2 5 Hd Reagent Kit Red, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope%C2%AE+2%2E5+hd+detection+reagent-red+kit/RNAscope+2%2E5+HD+Detection+Reagents-RED/pmc13201094-85-7-12
    Average 99 stars, based on 1 article reviews
    rnascope 2 5 hd reagent kit red - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    (A) Depletion of Fto in the NAcSh using microinjected AAV-Cre/GFP. RNAscope with Fto probe and Fto/GFP imaging was performed on brain sections from Fto fl/fl mice microinjected with AAV-Cre/GFP or AAV-GFP in the NAcSh after morphine CPP study, as described in the Methods. AAV-Cre: AAV5-CMV-Cre/GFP; AAV-GFP: AAV5-CMV-GFP; core: NAc core; shell: NAc shell (NAcSh). (B) Schematic of CPP. (C) Reduced morphine CPP by disrupting Fto in the NAcSh via AAV-Cre in Fto fl/fl mice. Fto fl/fl mice were bilaterally microinjected in the NAcSh with AAV-Cre/GFP or AAV-GFP. Morphine CPP was performed as shown in five weeks post-injection. **: p < 0.01, two-tailed Student t -test. (D) No significant changes in morphine CPP by disrupting both Fto and Pcif1 in the NAcSh in Fto fl/fl ; Pcif1 fl/fl mice. Fto fl/fl ; Pcif1 fl/fl mice were microinjected in the NAcSh with AAV-Cre/GFP or AAV-GFP. Morphine CPP was performed as shown in five weeks post-injection. n.s.: No statistical significance, two-tailed Student t -test. (E) No significant changes in morphine CPP by disrupting Pcif1 in the NAcSh in Pcif1 fl/fl mice. Pcif1 fl/fl mice were microinjected in the NAcSh with AAV-Cre/GFP or AAV-GFP. Morphine CPP was performed as shown in five weeks post-injection. p = 0.1024, no statistical significance, two-tailed Student t -test.

    Journal: bioRxiv

    Article Title: Reversible m 6 Am methylation of snRNA by FTO controls morphine reward and tolerance without altering analgesia

    doi: 10.64898/2026.08.06.743062

    Figure Lengend Snippet: (A) Depletion of Fto in the NAcSh using microinjected AAV-Cre/GFP. RNAscope with Fto probe and Fto/GFP imaging was performed on brain sections from Fto fl/fl mice microinjected with AAV-Cre/GFP or AAV-GFP in the NAcSh after morphine CPP study, as described in the Methods. AAV-Cre: AAV5-CMV-Cre/GFP; AAV-GFP: AAV5-CMV-GFP; core: NAc core; shell: NAc shell (NAcSh). (B) Schematic of CPP. (C) Reduced morphine CPP by disrupting Fto in the NAcSh via AAV-Cre in Fto fl/fl mice. Fto fl/fl mice were bilaterally microinjected in the NAcSh with AAV-Cre/GFP or AAV-GFP. Morphine CPP was performed as shown in five weeks post-injection. **: p < 0.01, two-tailed Student t -test. (D) No significant changes in morphine CPP by disrupting both Fto and Pcif1 in the NAcSh in Fto fl/fl ; Pcif1 fl/fl mice. Fto fl/fl ; Pcif1 fl/fl mice were microinjected in the NAcSh with AAV-Cre/GFP or AAV-GFP. Morphine CPP was performed as shown in five weeks post-injection. n.s.: No statistical significance, two-tailed Student t -test. (E) No significant changes in morphine CPP by disrupting Pcif1 in the NAcSh in Pcif1 fl/fl mice. Pcif1 fl/fl mice were microinjected in the NAcSh with AAV-Cre/GFP or AAV-GFP. Morphine CPP was performed as shown in five weeks post-injection. p = 0.1024, no statistical significance, two-tailed Student t -test.

    Article Snippet: RNAscope was performed on sections using RNAscope Kit (ACD, Catalog #: 322360) with Fto probe (Advanced Cell Diagnostics (ACD), Catalog #: 434261) and/or Pcif1 probe (ACD, Catalog #: 157913), as well as Opal fluorophores (Akoya Biosciences), in ACD HybEZ II Hybridization System, following the manufactural protocol.

    Techniques: RNAscope, Imaging, Injection, Two Tailed Test

    (A) Depletion of Fto in the DRG sensory neurons using tamoxifen in Fto fl/fl ;AvCreERT2 mice. Tamoxifen (75 mg/kg) or Corn oil (Vehicle) was i.p. administered daily in Fto fl/fl ;AvCreERT2 mice for 10 days. RNAScope combined with IHC with anti-NeuN antibody was performed on the DRG sections from perfused mice (4% PFA) after morphine tolerance study, as described in the Methods. (B & C) Depleting Fto in the DRG sensory neurons attenuated morphine tolerance in Fto fl/fl ;AvCreERT2 mice. Morphine analgesia was measured by using a radiant heat tail-flick assay and quantified as the percentage of maximum possible effect (% MPE) as described in the Methods. Morphine dose-response curves (1, 3,10 and 30 mg/kg, s.c.) were measured on Days 1 and 5 with single dose (10 mg/kg, s.c.) on Day 3. The ED 50 values were calculated using non-linear regression analysis (GraphPad Prism 10) and listed on . B: Time course; C: Dose-response curve. **: p < 0.01; ***: p < 0.001, 2-way ANOVA with Bonferroni’s post hoc test. (D) Depleting Fto in the DRG sensory neurons had no effect on morphine CPP in Fto fl/fl ;AvCreERT2 mice. Morphine CPP was performed in Fto fl/fl ;AvCreERT2 mice treated with tamoxifen or vehicle same as . Preference (Difference Score) was calculated as difference between the times spent in the morphine-paired chamber minus time spent in the saline-paired chamber on the post-test day (day 6) and the pre-test day (day 1). n.s: no statistical significance, two-tailed Student t -test. (E & F) Depleting Fto in the NAcSh had no significant effect on morphine tolerance in Fto fl/fl mice. Morphine tolerance in Fto fl/fl mice microinjected with AAV-Cre or AAV-GFP was performed same as B (E, time course) & C (F, dose-response curve). No statistical significance, 2-way ANOVA with Bonferroni’s post hoc test.

    Journal: bioRxiv

    Article Title: Reversible m 6 Am methylation of snRNA by FTO controls morphine reward and tolerance without altering analgesia

    doi: 10.64898/2026.08.06.743062

    Figure Lengend Snippet: (A) Depletion of Fto in the DRG sensory neurons using tamoxifen in Fto fl/fl ;AvCreERT2 mice. Tamoxifen (75 mg/kg) or Corn oil (Vehicle) was i.p. administered daily in Fto fl/fl ;AvCreERT2 mice for 10 days. RNAScope combined with IHC with anti-NeuN antibody was performed on the DRG sections from perfused mice (4% PFA) after morphine tolerance study, as described in the Methods. (B & C) Depleting Fto in the DRG sensory neurons attenuated morphine tolerance in Fto fl/fl ;AvCreERT2 mice. Morphine analgesia was measured by using a radiant heat tail-flick assay and quantified as the percentage of maximum possible effect (% MPE) as described in the Methods. Morphine dose-response curves (1, 3,10 and 30 mg/kg, s.c.) were measured on Days 1 and 5 with single dose (10 mg/kg, s.c.) on Day 3. The ED 50 values were calculated using non-linear regression analysis (GraphPad Prism 10) and listed on . B: Time course; C: Dose-response curve. **: p < 0.01; ***: p < 0.001, 2-way ANOVA with Bonferroni’s post hoc test. (D) Depleting Fto in the DRG sensory neurons had no effect on morphine CPP in Fto fl/fl ;AvCreERT2 mice. Morphine CPP was performed in Fto fl/fl ;AvCreERT2 mice treated with tamoxifen or vehicle same as . Preference (Difference Score) was calculated as difference between the times spent in the morphine-paired chamber minus time spent in the saline-paired chamber on the post-test day (day 6) and the pre-test day (day 1). n.s: no statistical significance, two-tailed Student t -test. (E & F) Depleting Fto in the NAcSh had no significant effect on morphine tolerance in Fto fl/fl mice. Morphine tolerance in Fto fl/fl mice microinjected with AAV-Cre or AAV-GFP was performed same as B (E, time course) & C (F, dose-response curve). No statistical significance, 2-way ANOVA with Bonferroni’s post hoc test.

    Article Snippet: RNAscope was performed on sections using RNAscope Kit (ACD, Catalog #: 322360) with Fto probe (Advanced Cell Diagnostics (ACD), Catalog #: 434261) and/or Pcif1 probe (ACD, Catalog #: 157913), as well as Opal fluorophores (Akoya Biosciences), in ACD HybEZ II Hybridization System, following the manufactural protocol.

    Techniques: RNAscope, Tail Flick Test, Saline, Two Tailed Test